Translational Bioimaging Core

This is unpublished

Mission

Brain image with pathology correlation.The Translational BioImaging Core Shared Resource (TBICSR) provides state of the art in vivo imaging equipment and expertise to support the diverse array of Consortium member preclinical research projects. TBICSR strives to provide easy access to a wide array of imaging modalities including magnetic resonance imaging (MRI), positron emission tomography (PET), computed tomography (CT), ultrasound (US), and optical imaging in a cost effective and efficient manner. Due to its multi-expert, multi-institutional organization, TBICSR bolsters the research objectives of Consortium Users through personnel with knowledge specific to each imaging modality and relevant in vivo biomarkers.  TBICSR imaging modality experts, known as consultants in TBICSR, and imaging specialists direct Consortium users to the proper image modality through consultation and education.  TBICSR staff foster animal imaging studies by providing scientific imaging and animal protocol development services, direct preclinical data acquisition, and image analysis and interpretation.

Services

Vascular images.The TBIC supports and facilitates imaging research for members of the Cancer Consortium using resources available at Fred Hutch and UW in collaboration with multi-disciplinary imaging research groups specialized in respective imaging technologies. The TBIC provides services including:

  • Support for animal imaging using equipment available to the Consortium

  • Consultation for the effective use of imaging for specific research projects

  • Training and education in imaging research

  • Facilitation of the development of new imaging technologies

  • Infrastructural support for animal imaging research

  • Strategic acquisition of new imaging devicesFDG PET/CTFDG PET/CT

FDG PET/CT.

Resources

The TBIC Core resources are allocated at Fred Hutch. Other resources are accessible through centers, shared resources, and laboratories in the Department of Radiology, Washington National Primate Research Center, and the Department of Radiation Oncology.

Modality

Equipment

Location

Optical

IVIS Spectrum (x 2)

Fred Hutch (FH)
 

IVIS Spectrum*

FH

 

Lago-X*

FH

 

IVIS Spectrum (x2)

UW South Lake Union (SLU)

 

IVIS Spectrum*

UW Animal Research and Care Facility (ARCF)

Cryo-Fluorescence Tomography

Xerra*

UW ARCF

Microscopy

Two-photon Zeiss microscope + OPO

FH

Ultrasound

Visual Sonics Vevo F2 ultrasound*

FH
 

Aixplorer/Verasonic software

UW

X-Ray

MX-20 Specimen Radiograph

FH

Magnetic Resonance Imaging (MRI) and Magnetic Resonance Spectroscopy (MRS)

Philips 3T Achieva (x 2)

UW SLU and UW Medical Center
 

7T/3T MRI MRS*

FH
 

4.7T Varian MRI/MRS

UW
 

14T MRI/MRS

UW SLU

Positron Emission Tomography (PET)

Hamamatsu PET

UW

Positron Emission Tomography/Computed Tomography (PET/CT)

Siemens Inveon PET/CT

UW ARCF

External Beam Radiation Therapy (EBRT) with CT

xstral Small Animal Radiation Research Platform (SARRP) plus small animal Proton Beam

UW Radiation Oncology

Computed Tomography (CT)

Quantum GX2 micro-CT*

FH

Body Composition Measurement

EchoMRI*

FH
Stem Cell Tracking.

CoNTACTS

Study Set Up and Budget Issues

Robert Miyaoka, PhD

Director, UW - Fred Hutch TBIC, UW Radiology

(206) 543-2084

rmiyaoka@uw.edu

Institutional Operations and UW/Fred Hutch Cancer Consortium

Elena Carlson, MS

Fred Hutch Preclinical Imaging Manager

(206) 667-4021

ecarlson@fredhutch.org

Study Set Up and Operations

Teri Blevins, BS, LVT, LAT

UW Imaging Specialist, Inter-Institutional Study Facilitator (IISF)

(206) 543-3653

tblev@uw.edu

Other TBIC Core Members

Donghoon Lee, PhD

UW Department of Radiology

Research Professor

Consultant: MRI/S

dhoonlee@uw.edu

Mark Muzi, MS

UW Department of Radiology

Consultant: Image Analysis

muzi@fredhutch.org

Neal Paragas, PhD

UW Department of Radiology

Research Associate Professor

Consultant: Optical and Multimodality Imaging

paragas@uw.edu

Cu64 PET.

Animal Housing and Transportation

Fred Hutch Comparative Medicine Director

Gordon Roble, DVM, MBA, DACLAM

206-667-5313

groble@fredhutch.org

Fred Hutch Animal Housing and Transportation Support

Amber Carte, BS, CMAR, RLATG

206-667-4184

acarte@fredhutch.org

UW Animal Housing Support

Susan C. Dowling, DVM

206-221-3933

sdowling@uw.edu

UW Transportation Support

Teri Blevins, BS, LVT, LAT

UW Imaging Specialist, Inter-Institutional Study Facilitator (IISF)

206-543-3653

tblev@uw.edu

IACUC

Fred Hutch Institutional Review Office
IACUC

Karen Hansen

206-667-4867

khansen@fredhutch.org

UW Office of Animal Care

206-685-7363

oawrss@uw.edu

FAQ

I am interested in imaging, but do not know where to start.

Start by contacting Teri Blevins, TBIC Imaging Specialist, (tblev@uw.edu or 206-543-3653) for initial consultation. If you know what imaging modality that you would like to use, she will facilitate a meeting with the director of that imaging resource. If you do not know what imaging modality you would like to use, she will set up a meeting with TBIC personnel to provide guidance on the best resource for your needs.

Can I do my own imaging?

At FHCRC, the optical, ultrasound, echo MRI and multiphoton intravital microsope can be user operated after appropriate training has been completed. The MRSolutions 7T/3T MRI is operated with the assistance of an imaging specialist.

Can I practice on a live animal?

In general, training is conducted on phantoms, but it is possible to use live animals for training with IACUC approval.

What if my animals are housed at UW, but my collaborators and the imaging equipment are at FHCRC (or vice versa)?

Inter-institutional imaging studies can be easily arranged. Contact Teri Blevins, TBIC Imaging Specialist, (tblev@uw.edu or 206-543-3653) for assistance with UW questions. Contact Elena Carlson, MS, (ecarlson@fredhutch.org or 206.667.4021) for assistance with FHCRC questions.

Does my imaging procedure need to be approved?

All protocols involving animals including: what you want to accomplish, what species you will work with, what procedures you would like to perform, who will perform the procedures, and the locations where you will perform the procedures much be approved by the IACUCs of the institutions involved. For assistance, contact Teri Blevins, TBIC Imaging Specialist, (tblev@uw.edu or 206-543-3653). 

If my study includes inter-institutional imaging, do I need to submit two IACUC applications?

No. UW and FHCRC IACUCs engage in cooperative review of each other’s protocols. You need only submit the protocol application to your home institution’s IACUC.

What if my animals are housed at UW, but my collaborators and the imaging equipment are at FHCRC (or vice versa)?

Inter-institutional imaging studies can be easily arranged. Contact Teri Blevins, TBIC Imaging Specialist, (tblev@uw.edu or 206-543-3653) for assistance with UW questions. Contact Elena Carlson, MS, (ecarlson@fredhutch.org or 206.667.4021) for assistance with FHCRC questions.

I have an idea for a pilot study; is funding available?

TBIC staff will assist investigators with Cancer Consortium pilot proposals that use imaging equipment. Contact the head of the specific resource for details in regards to collection of pilot data.

What does imaging cost?

UW and FHCRC each maintain tiered rate structures, with separate rates for internal and external users. Hourly rates for the various imaging devices and services are detailed below:

Imaging Cost

For up to date pricing, please contact the appropriate resource contact.

Imaging Modality

Internal Cost per hour

Cancer Consortium Member cost per hour

Location

Vevo 2100 and Vevo F2 Ultrasound

$35

$49

Fred Hutch SR

IVIS

$70

$96.60

Fred Hutch SR

Inveon PET/CT

PET tracers

$288

Actual cost + $35

$352

Actual cost + $35 + 15.6%

UW ARCF B141B

MR Solutions 7T/3T cryogen free MRI

$55

$111

Fred Hutch SR

14T MRI/S

$170

$200

UW SLU North N-101

Emit Imaging Xerra micro Cryo-Fluorescence Tomography System

TBD

TBD

UW ARCF B141C

Multiphoton intravital microscope

$55

$76

Fred Hutch SR

PerkinElmer Quantum GX2 microCT

$80

$111

Fred Hutch SR

Echo MRI

$28

$38

Fred Hutch SR

Xstral Small Animal Radiation Research Platform (SARRP) plus small animal Proton Beam system

TBD

TBD

UW

Fred Hutch User Imaging at Fred Hutch

Fred Hutch User Imaging at Fred Hutch.

For general assistance or questions please contact Elena Carlson, MS, (ecarlson@fredhutch.org or 206-667-4021).

All steps refer to a minimum time frame for approval. The approval process for each step could take up to several weeks longer.

  1. IACUC Application for Imaging:

    IACUC application: imaging approval
    Imaging specifications are required on your IACUC protocol.  It must include the following:
    (1) imaging devices and procedures to be performed
    (2) agents to be administered (ie. contrast, anesthetics)
    (3) personnel performing the imaging
    (4) number of animals
    (5) imaging frequency, session duration, maximum number of sessions, and interval between sessions.

  2. Fred Hutch – Complete Onboarding Process
    Contact Human Resources for information on obtaining a badge.

  3. Fred Hutch Access and Training:

    Imaging Specialist:  Brianna Wrightson (bwrights@fredhutch.org) or Elena Carlson, MS (ecarlson@fredhutch.org, 206-667-4021)

    3a.          Complete Facility Training

    For Facility Training contact Rodentservices@fredhutch.org.

    3b.          Complete Device-Specific Training

    Contact Imaging Specialist Brianna Wrightson (bwrights@fredhutch.org) or Elena Carlson, MS (ecarlson@fredhutch.org, 206-667-4021) to request training availability.

    Vevo 2100 and Vevo F2 Ultrasound
    IVIS Optical
    Lago-X Optical
    MR Solutions 7T/3T
    Multiphoton Intravital Microscope
    PerkinElmer Quantum GX2 microCT
    EchoMRI

  4. Reserve Imaging Device

    Reserve imaging device on the web based Shared Resources calendar Scheduler. Secure connection by access being on the Hutch Campus or VPN required to access link to the calendar located at this URL:

    https://fom.fhcrc.org/fom/welcome

Fred Hutch User Imaging at UW

FH User Imaging at UW.

For general assistance or questions please contact Contact Teri Blevins, TBIC Imaging Specialist, (tblev@uw.edu or 206-543-3653).

All steps refer to a minimum time frame for approval. The approval process for each step could take up to several weeks longer.

  1. IACUC Application for Imaging

    IACUC application: imaging approval
    Imaging specifications are required on your IACUC protocol.  It must include the following:
    (1) imaging devices and procedures to be performed
    (2) agents to be administered (ie. contrast, anesthetics)
    (3) personnel performing the imaging
    (4) number of animals
    (5) imaging frequency, session duration, maximum number of sessions, and interval between sessions.
    (6) After IACUC approval: confirm budget with UW billing department

  2. UW Imaging-Specific Access and Training

    Bruker 14T MRI
    Contact Donghoon Lee (dhoonlee@uw.edu) for Bruker 14T use.

    Inveon Small Animal PET-CT
    (1) Submit PET-CT Project Application Form
    (2) Complete Radiation Safety Training (3) Obtain dosimeter badge and ring
    (4) Schedule device use
    Contact Robert Miyaoka (rmiyaoka@uw.edu) or Teri Blevins (tblev@uw.edu) for Inveon scheduling.

    Contact Swati Rane Levendovszky (srleven@uw.edu) for Philips 3T MRI assistance and scheduling.

  3. Arranging Animal Transfer

    (1) Submit Imaging Animals at the University of Washington Request Form to import@uw.edu
    Contact Teri Blevins, (tblev@uw.edu) for assistance filling out and submitting Imaging Request Form.
    (2) Submit Fred Hutch Small Animal shipping application
    Contact Amber Carte (acarte@fredhutch.org) for assistance.

  4. Transportation

    (1) Option 1: Submit DCM Vehicle Transportation Service Request Form to cruisin@uw.edu
    OR Option 2: Submit request to rodentservices@fredhutch.org
    (2) Arrange for Comp. Med. to load animals
    (3) Arrange for personnel to receive animals at UW
    Transport fees differ depending on option 1 or option 2
    Contact Teri Blevins, (tblev@uw.edu) for assistance with arranging transportation.

UW User Imaging at UW

UW User Imaging at UW.

For general assistance or questions please contact Teri Blevins (tblev@uw.edu).

All steps refer to a minimum time frame for approval. The approval process for each step could take up to several weeks longer.

  1. IACUC Application for Imaging

    IACUC application: imaging approval
    Imaging specifications are required on your IACUC protocol.  It must include the following:
    (1) imaging devices and procedures to be performed
    (2) agents to be administered (ie. contrast, anesthetics)
    (3) personnel performing the imaging
    (4) number of animals
    (5) imaging frequency, session duration, maximum number of sessions, and interval between sessions.
    (6) After IACUC approval: confirm budget with UW billing department

  2. UW Imaging-Specific Access and Training

    Bruker 14T MRI/S
    Contact Donghoon Lee (dhoonlee@uw.edu) for Bruker 14T use.

    Inveon Small Animal PET-CT
    (1) Submit PET-CT Project Application Form
    (2) Complete Radiation Safety Training
    (3) Obtain dosimeter badge and ring
    (4) Schedule device use
    Contact Robert Miyaoka (rmiyaoka@uw.edu) or Teri Blevins (tblev@uw.edu) for Inveon scheduling.

UW User Imaging at Fred Hutch

UW User Imaging at FH.

For general assistance or questions please contact Elena Carlson, MS, (ecarlson@fredhutch.org or 206-667-4021).

All steps refer to a minimum time frame for approval. The approval process for each step could take up to several weeks longer.

  1. IACUC Application for Imaging:

    IACUC application: imaging approval
    Imaging specifications are required on your IACUC protocol.  It must include the following:
    (1) imaging devices and procedures to be performed
    (2) agents to be administered (ie. contrast, anesthetics)
    (3) personnel performing the imaging
    (4) number of animals
    (5) imaging frequency, session duration, maximum number of sessions, and interval between sessions.

  2. Fred Hutch – Complete Onboarding Process

    Contact Human Resources for information on obtaining a badge.

  3. Fred Hutch Access and Training

    Imaging Technician:  Brianna Wrightson (bwrights@fredhutch.org) or Elena Carlson, MS (ecarlson@fredhutch.org, 206-667-4021).

    3a.          Complete Facility Training

    For Facility Training contact rodentservices@fredhutch.org.

    3b.          Complete Device-Specific Training

    Contact Imaging Technician Brianna Wrightson (bwrights@fredhutch.org) or Elena Carlson, MS (ecarlson@fredhutch.org, 206-667-4021).

    Vevo 2100 Ultrasound
    IVIS Bruker 1T MRI
    2-Photon Microscope

  4. Arranging Animal Transfer

    (1) Submit UW Request for Rodent Health Monitoring Report to export@uw.edu
    Contact Teri Blevins, (tblev@uw.edu) for assistance filling out and submitting this form.
    (2) Submit Fred Hutch Receiving Application
    Contact Amber Carte (acarte@fredhutch.org) for assistance.

  5. Reserve Imaging Device

    Reserve imaging device on the web-based Shared Resources calendar scheduler. Secure connection by being on Hutch campus or VPN required to access link to calendar located at this URL:

    https://fom.fhcrc.org/fom/welcome

  6. Transportation

    (1) Option 1: Submit DCM Vehicle Transportation Service Request Form to cruisin@uw.edu
    OR Option 2: Submit request to rodentservices@fredhutch.org
    Transport fees differ depending on option 1 or option 2. Contact Teri Blevins (tblev@uw.edu) for assistance with arranging transportation.
    (2) Arrange for personnel to load animals at UW
    Contact Amber Carte (acarte@fredhutch.org) for assistance with receiving animals at Fred Hutch.